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Lecture 13

D103 - Lecture 13 - Exocytosis.docx

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Department
Biological Sciences
Course Code
BIO SCI D103
Professor
Grant Mac Gregor

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A. Clicker
a. Sorting signal that directs exit of a soluble ECM component from the ER
i. Interact indirectly with the cytosolic vesicle coat at the ER
b. Dealing with the primy AA of a soluble protein that is targeted to the
lyosome
i. Lyosome
1. M6P
a. Requires a glycan
b. Requires a consensus sequence for glycosylation
i. Occurs into the ER
1. Requires hydrophobic sorting
sequence
2. N-terminus sorting signal
ii. Asn-X-Ser/Thr
iii. N-terminal hydrophobic signal sequence
b. Soluble protein
i. Doesn't have positive charges
B. Secretory Pathway
a. TGN
i. Trans-golgi network
b. All transport steps involve vesicles
c. Topology will not change throughout the transport pathway
d. Not linked with cytosolic organelles
i. (mito, nucleus)
B. Golgi Apparatus
a. Stacked and flat
i. Stacked/perinuclear position are not conserved
b. interconnected
c. Cis/medial/trans cisterna
i. Differ in the protein, and lipid composition
b. Stacking is not required for protein transport
c. Disrupting perinuclear Golgi position
i. Interrupts the leading edge
1. Check recording8:30
B. N-glycans
a. Processed as they transition through the cisternae
b. N-glycosylation
i. ER: Quality control
ii. Cis-Golgi: generation of the lysosomal sorting signal
1. Mannose 6 phosphate
ii. Plasma membrane
1. Protection
2. interaction
B. O-glycosylation
a. Attachment of GalNAc + oligosaccharide to Thr/Ser
b. Only occurs in the Golgi
c. Single GalNAc as the linker
i. N-Acetylgalactosamine
b. Proteoglycans

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Description
A. Clicker a. Sorting signal that directs exit of a soluble ECM component from the ER i. Interact indirectly with the cytosolic vesicle coat at the ER b. Dealing with the primy AA of a soluble protein that is targeted to the lyosome i. Lyosome 1. M6P a. Requires a glycan b. Requires a consensus sequence for glycosylation i. Occurs into the ER 1. Requires hydrophobic sorting sequence 2. N-terminus sorting signal ii. Asn-X-Ser/Thr iii. N-terminal hydrophobic signal sequence b. Soluble protein i. Doesn't have positive charges B. Secretory Pathway a. TGN i. Trans-golgi network b. All transport steps involve vesicles c. Topology will not change throughout the transport pathway d. Not linked with cytosolic organelles i. (mito, nucleus) B. Golgi Apparatus a. Stacked and flat i. Stacked/perinuclear position are not conserved b. interconnected c. Cis/medial/trans cisterna i. Differ in the protein, and lipid composition b. Stacking is not required for protein transport c. Disrupting perinuclear Golgi position i. Interrupts the leading edge 1. Check recording8:30 B. N-glycans a. Processed as they transition through the cisternae b. N-glycosylation i. ER: Quality control ii. Cis-Golgi: generation of the lysosomal sorting signal 1. Mannose 6 phosphate ii. Plasma membrane 1. Protection 2. interaction B. O-glycosylation a. Attachment of GalNAc + oligosaccharide to Thr/Ser b. Only occurs in the Golgi c. Single GalNAc as the linker i. N-Acetylgalactosamine b. Proteoglycans i. Sugars are attached to the core proteins b. Mucin-like proteins i. Found on 1. Mucins in mucus (airway cells) 2. Cell Surface proteins (blood groups) B. Blood groups a. Antibodies i. Antibodies will react with the antigens on the RBC 1. Causes aggregation of cells B. Cisternal Maturation a. Collagen is synthesized in the ER i. Very large proteins - fitting into small vesicles b. Vesicular transport model
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