BIO SCI D103 Lecture Notes - Missense Mutation, Microbiological Culture, Phase Transition
18 Mar 2014
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Studying g2 to m transition: budding, fusion i. Yeast: good model system, small genome size c. Clickers: mutation that would produce a protein with a temperature sensitive mutation i. (b) missense mutation, change the thr to his. Can"t put a phosphate on the threonine (gas, phosphorylated by i. ii. Doesn"t require creation of a homozygous recessive i. ii. Temperature sensitive (ts: conditional mutant, only see the mutation effect under a certain temperature c. Incubate for a period of time after mutation. Add mutagen to yeast liquid culture i. ii. Allow the mutation to become fixed in the genome. Plate the mutated yeast onto solid media iii. Colonies will grow (each from a single yeast cell) iv. v. Propagate the missing colony from the b. permissive plate. Isolation of cell cycle mutants: premature entry i. Shows that the cdna introduced fixed the mutated. Show that the machinery between frogs and yeast are the same.
