Bio 97 Peer Tutor W2 .doc

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Department
Biological Sciences
Course
BIO SCI 97
Professor
Rahul Warrior
Semester
Fall

Description
Tutor: Christina Phan Week 2 Worksheet Bio 97: Warrior/Cinquin/Bardwell OH: Wednesday, 12-2 PM, SH 128 [email protected] 1. What are the components of a DNA nucleotide? What is the difference between the nucleotide of a DNA and that of RNA? DNA RNA Thymine Uracil 2’ H 2’ OH H-bonds (double stranded) Single stranded 2. DNA synthesis occurs in the: 5’  3’ direction DNA polymerase is an enzyme that extends a DNA chain, takes the individual nucleotide triphosphates and joins them together to make a long DNA polymer. The 5’ end of the DNA has a phosphate group on it while the 3’ end has a hydroxyl group on it. The way it extends the chain is by incorporating a nucleotide triphosphate at the 3’ end of the DNA; the 3' hydroxyl attaches to the triphosphate group of the nucleotide triphosphate, liberating pyrophosphate to form a phosphodiester bond. The Tutor: Christina Phan Week 2 Worksheet Bio 97: Warrior/Cinquin/Bardwell OH: Wednesday, 12-2 PM, SH 128 [email protected] pyrophosphate is then hydrolyzed. The energy of the hydrolysis is the energy used in making the DNA molecule/chain. Dideoxynuelotide  termination of elongation 3. You are analyzing a piece of DNA from an unknown organism and see that there is only one origin of replication. What kind of organism is this? Prokaryote Eukaryote: multiple origin replication Replication only occurs in the S-phase of interphase cycle 4. Label each protein involved in DNA replication and label the leading and lagging strand. Leading Lagging strang DNA polymerase III: main one that synthesizes the DNA DNA polymerase I: removes the RNA primer from the Okazaki fragments Match each of the proteins with its function: Gyrase: D RNA primase: A Helicase: F DNA ligase: C SSB proteins: B DNA polymerase: E a. primes DNA synthesis Tutor: Christina Phan Week 2 Worksheet Bio 97: Warrior/Cinquin/Bardwell OH: Wednesday, 12-2 PM, SH 128 [email protected] b. prevent single c. joins gaps once RNA primase is removed strands from hybridizing with each d. relieves torsional stress when helicase unzips DNA other e. synthe
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