D103 - Lecture 5 - Cytoskeleton.docx

3 Pages
73 Views
Unlock Document

Department
Biological Sciences
Course
BIO SCI D103
Professor
Grant Mac Gregor
Semester
Winter

Description
A. Clicker a. Experimentally determine which component of the cytoskeleton is responsible for cell migration? i. Disrupt each stucture separately ii. Each answer - control experiment b. Loss of profilin B. Mitochondria a. Power plant of the cell b. Maternal inheritance i. Pedigree problem! B. Size comparison! C. Cytoplasmic Inheritance a. Normal cell division i. Mutated nucleoids 1. Progeny contains different mixtures of healthy/mutated mitochondria 2. Phenotype also depends on the identity of the cell ii. Is it Possible to predict mitochondrial mutation pedigree b. Mito Mutations i. More pronounced in tissues with high energy requirement ii. Can occur in both the mtDNA and nDNA. b. Mitochondria produce 13 proteins, has 22 tRNA B. Cytoplasmic vs Maternal Inheritance? a. Cytoplasmic i. Somatic cells ii. Random segregation iii. Threshold b. Maternal i. germline B. Mitochondrial Diseases a. Heteroplasmy i. Mutant and wt forms of mtDNA in the same cell b. Threshold Effect i. Diseases may only be manifested if the % of mutant mtDNA reaches a level (different between tissues) 1. Same mtDNA mutation can make different phenotypes depending on location of cell B. Neutraphil a. Moving cell that chases after bacteria i. Possible due to the fluidity of membrane ii. Follows a chemical signal released by the bacteria B. Cytoskeleton a. Structure, stability b. Promotes movement within and of the cell B. Cytoskeletal Elements a. Microtubules i. MTOC = centrosome 1. Microtubule organizing center ii. Made of alpha and beta tubulin 1. Assembled into protofilaments a. 13 protofilaments makes 1 microtubule ii. Largest, 25nm iii. Intrinsically polar structure 1. Ends are different b. LOOK AT IMAGE - DETERMINE WHICH CYTOSKELETON IT IS c. Intermediate Filaments i. Middle Size, ii. Made of 1. Intermediate filament monomer a. Have bulky protein ends at the N and C terminals i. Polar structure ii. Dimer
More Less

Related notes for BIO SCI D103

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit