BIO SCI M125 Lecture 6: Lecture 6 Guide
21 Dec 2016
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Dna pol has 3" - 5" exonuclease that is specific and thus efficeint. Repressor is downstream of promoter while activator is upsreat. Use taq polymerase to synthesize strand while heating again. Goes through pcr cycle multiple times. (can be cut by ecori endonuclease and placed into cloning vector. ) When rna pol inserts an incorrect nucleotide , it stutters and allows for phorphoyrliss to be used. Processive - runs smoothly along strand until termination sequence. Rna pol is very error prone compared to dna pol. This can be tolerated because mulktiple copies of rna are made. Rna is advantageous for viruses because their error prone transcription allows them to escape immunity by host cells. Termination sequences tell when pol to start, release, and dissociated from temok. P protein moves along strand fro rut site and stalls when encountering transcription complex. Processive - pol sticks on to dna transciprt and keeps synthesizing until reacing termination sequnece.
