BCH 4054 Lecture Notes - Lecture 22: Serine Protease, Carbonic Anhydrase, Hydrogen Bond
Document Summary
Known how oxygen binds to myoglobin and hemoglobin. Know what amino acids are involved and how it happens. Make sure to know the diference between r and t state. Be sure to know how to draw happy amino acid chain on test 2. The secondary structure will have two beta turns because of the two prolines and is a type 1 hydrogen bond. Serine protease or carbonic anhydrase will be on the test also. Know which amino acids and numbers coordinate to the acive sites. Covalent catalysis will have groups that are highly polarized to form a bond between the enzyme and the nucleophile. Cofactors for metal ions will help a substrate orient for a reacion. Carbonic anhydrase can catalyze a reacion by using a metal ion (typically. Zn2+) to increase the acidity and deprotonate h2o to oh-. Zn2+ coordinates to three imidazoles and carbon dioxide to bind a new water molecule.