BMD RES 5HA Lecture Notes - Lecture 5: Cfu-Gemm, Lymphopoiesis, Functional Genomics
23 May 2018
School
Department
Course
Professor
Lecture 5
• Inverted repeat -> RNA fold back on itself -> hairpin (hpRNA)
• Complementary base pairing -> H bond -> siRNA bringing RISC complex bond mRNA ->
mRNA cleaved & degraded -> a’t traslate futioal protei
• RNAi great for functional genomics (large scale, reverse genetics)
• But does’t alays ork ell
- Too much mRNA
- If targeted ase pair ot iluded i a differet isofor, o’t e reogized
- If eak ehaer, o’t ake too ay hpRNA
- Whe RNA does’t fold properly ito hairpi
• Some lines work better than others
- If RNAi causes phenotype -> targeted gene likely has a function
- If RNAi does’t ause a pheotype -> no basis if gal4 is expressed
➢ Maybe RNAi is not working?
➢ Maybe RNAi is working -> gene has no function in cell
Hematopoiesis: human
• B cells make antibodies
• T cells
- Cytotoxin T cells interact with somatic cells & look for those infected by viruses
➢ Cells have proteins on surface representing things in the cell
➢ MHC complex: T cell finds MHC complex on cell surface -> kill the cell
• Precursors become mature cells
- Different precursors have different potentials
• 2 main lineages of blood cell in vertebrates
- lymphoid lineage & myeloid lineage
- common lymphoid progenitor -> lymphoid lineage
- common myeloid progenitor -> myeloid lineage
find more resources at oneclass.com
find more resources at oneclass.com
