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Class Notes for Biomedical Research at University of California - Los Angeles

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BMD RES 5HA Lecture Notes - Lecture 3: Circulatory System, Lymph Node, Heart
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model organisms: excellent genetic systems - Yeast, C. elegans, Drosophila, Zebrafish Experimentation & manipulation: - Eggs can be modified in Xenupus (frogs) & chickens Genomic analyses: - Fu...

Biomedical Research
BMD RES 5HA
Rafael Romero
BMD RES 5HA Lecture Notes - Lecture 4: Dna-Binding Domain, Tata Box, Heterochromatin
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Inhibiting an inhibition: disinhibition (repressor binds) Leg dev. Antp repressor --------------------------------------------------------------------------- How do we control ...

Biomedical Research
BMD RES 5HA
Rafael Romero
BMD RES 5HA Lecture Notes - Lecture 5: Cfu-Gemm, Lymphopoiesis, Functional Genomics
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Lecture 5 Inverted repeat -> RNA fold back on itself -> hairpin (hpRNA) Complementary base pairing -> H bond -> siRNA bringing RISC complex bond mRNA -> mRNA cleaved & degraded -> cant translate fun...

Biomedical Research
BMD RES 5HA
Rafael Romero
BMD RES 5HA Lecture Notes - Lecture 4: Polyacrylamide Gel Electrophoresis, Antibody, Reducing Agent
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Dr. J presentation The evidence Rna sequencing tells you how many times an rna is made When the cell undergoes starvation, the number of ribosomes decreases and the splicesosomes are now free to splice other things She det...

Biomedical Research
BMD RES 5HA
Rafael Romero
BMD RES 5HA Lecture Notes - Lecture 1: Cell Cycle, Dna Replication, Mitosis
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4.10.18 PremRNa splicing is a critical step of gene expression They want to understanding splicing to the highest in order to hopefully prevent and possibly control diseases Diseases usually occur from errors in splicing C...

Biomedical Research
BMD RES 5HA
Rafael Romero
BMD RES 5HA Lecture Notes - Lecture 2: H2Afz, Chromatin, Intron
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Yeast as a model organism Replica plating is used to screen colonies and identify mutations The colony that couldnt grow shows that it has a leucine mutation where it cannot create ...

Biomedical Research
BMD RES 5HA
Rafael Romero
BMD RES 10H Lecture Notes - Lecture 4: Antibody, Cytoskeleton, Intellectual Disability

BR​ ​5HA​ ​-​ ​Lec​ ​3​ ​(10/10/17) - People​ ​with​ ​small​ ​duplication​ ​in​ ​15q11-13​ ​have​ ​an​ ​extra​ ​copy​ ​of​ ​about​ ​a​ ​dozen​ ​genes - Some​ ​regions​ ​in​ ​chromosomes​ ​are​ ​weaker​ ​(b...

Biomedical Research
BMD RES 10H
Stan Romero
BMD RES 10H Lecture Notes - Lecture 3: Tsc1, Blind Luck, Mecp2

BR​ ​5HA​ ​-​ ​Lec​ ​2 ❖ Alters​ ​the​ ​function​ ​of​ ​one​ ​gene ❖ Studying​ ​autism​ ​base​ ​on​ ​one​ ​gene ❖ What​ ​causes​ ​autism?​ ​How​ ​to​ ​prevent​ ​them? ❖ Cell​ ​culture​ ​also​ ​considers​ ​a​ ​model​ ​org...

Biomedical Research
BMD RES 10H
Stan Romero
BMD RES 10H Lecture Notes - Lecture 2: Intellectual Disability, Developmental Disorder, Fear Conditioning

Hao Nguyen UID: 904830467 October 5, 2017 BR 5HA Fall 2017 – Initial Seminar Report 1 1) I have completed the survey. 2) Dr. Kugler’s lab focuses on the study of Dup15q duplication syndrome whi...

Biomedical Research
BMD RES 10H
Stan Romero
BMD RES 5HA Lecture Notes - Lecture 15: Chromosome, Transposable Element, Cell Adhesion Molecule
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Heat Maps • Heat map is a color representation of large amounts of quantitative data ◦ A format that allows people to quickly grasp the point • Taking away the numbers can be even more pleasing to ...

Biomedical Research
BMD RES 5HA
Robert Romero
BMD RES 5HA Lecture Notes - Lecture 16: Transposase, Spliceosome, Fusion Protein
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MiMIC • MiMIC: Minos Mediated Integration Cassettes • Cross the fly with just the transposon ends and the fly with just the transposase ◦ The offspring will have both the transposase and the element ...

Biomedical Research
BMD RES 5HA
Robert Romero
BMD RES 5HA Lecture Notes - Lecture 18: Myofascial Trigger Point, Chromosome, Fusion Protein
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• So far we have been able to observe which lamina neurons are close to which medullary neurons ◦ But how can we know if they're really connected? • By looking for synaptic connections • C...

Biomedical Research
BMD RES 5HA
Robert Romero
BMD RES 5HA Lecture Notes - Lecture 17: Flippase, Multicolor, Repressor Lexa
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• To help us identify the layers within the medulla, antibody staining of the receptor cell axons (in red, R7 and R8) was also provided ◦ Like a landmark • In green: the expression pattern of 6 different ...

Biomedical Research
BMD RES 5HA
Robert Romero
BMD RES 5HA Lecture Notes - Lecture 19: Midbrain, Antibody, Zygosity
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CRISPR-Cas9 • You need the right Cas9 for the right path • Tracer RNA matches the repeat to stabilize the structure ◦ If the tracer isn't around, none of the process works • The Cas9 cleaves both strands of DNA ...

Biomedical Research
BMD RES 5HA
Robert Romero
BMD RES 5HA Lecture Notes - Lecture 14: Polymerase Chain Reaction, Reverse Transcriptase, Repressor Lexa
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Basic Gal4-UAS system • On one DNA, there's a tissue specific promoter (L3 neuron specific for this experiment), followed by a Gal4 gene (transcription factor—comes from yeast) • On another DNA, there's a UAS ...

Biomedical Research
BMD RES 5HA
Robert Romero

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