GE CLST M1A Lecture Notes - Lecture 22: Quinolone, Dna Extraction, Ceftiofur
25 May 2017
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Document Summary
The development of antimicrobials over the 20th century lead to incredible improvements in medical care. Ifap impacts to public health (how antibiotics work: pathogens are routinely exposed to antibiotics, susceptible pathogens die, resistant pathogens survive, resistant pathogens multiply. This is called horizontal gene transfer: contact between cells, plasmids can travel through a channel. Involves viruses carrying pieces of dna from cell to cell: resistance may continue even after antimicrobials are no longer present. Other substances can apply co-resistance pressure: when bacteria are stressed by one contaminant, such as metals, their resistance mechanism to that stressor result in result in resistance to other stressors, including antibiotics. We have been collecting bacteria in air from agricultural and control sites around ca. We purify bacterial isolates and test them for antibiotic resistance. We extract dna and test for bacteria and antibiotic resistance genes. Many isolates were resistant to multiple antibiotics. There are 1200 bacteria isolates that were catalogued.
