MIMG 101 Lecture Notes - Lecture 20: Petri Dish, Nystatin, Cell Envelope

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Examples of additional developmental programs resting body spore formation formation of biofilm. Antibiotic production: likely evolved to compete against other microbes growing and using substrate. Can affect single cell or community of cells. Streptomyces (gram+ soil bacteria) i. e. streptomycin, tetracycline, nystatin, erythromycin, penicillin, neomycin (step nn) Produces antibiotics: targets: ribosome (streptomycin, tetracycline), cell membrane (nystatin), cell wall biosynthesis (erythromycin, antibiotics bind to ribosome active site -> peptide elongation prevented, unable to bind/affect euk ribosomes. Stages: vegetative substrate cell, aerial tube (differentiated state), resting cell spore: each controlled by different sub-program, each have different metabolic state, each occurs in diff stage of life cycle. Testing antibiotic sensitivity: take sample on sterile filter disk and test w/ diff antibiotics on petri dish, observe zone of clearing (effective antibiotic) and no clearing. Some cells programmed to form spores and hibernate: not all can become spores, but all can give rise to a vegetative cell (not one way/committed- is flexible!)

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