MIMG 101 Lecture Notes - Lecture 22: Human Microbiota, Enterococcus, Rumen
Document Summary
Each region has own microbiome w/ distinct 16s sequences. Bacteria digestion contribute to 10-15% of human caloric intake: 80% for horse, 95% for cows. Human microbiome examination strain differences assigned by dna sequencing: collect sample (poop, extract community dna from sample, sequence w pcr primers, sort 16s sequences, add up amount of each + compare. 16% undigested food, 75% water, some human cells. Not indicative of gut microbes b/c diff microbes in diff regions. Varies ph and oxygen levels in stomach, si, li: not homogenous, anaerobic microbes. Poop is 1/3 bacteria, 2/3 undigeted stuff. Different major bacteria present in each region. Intestine = cow rumen: lactobacilli stomach, few other bacteria b/c acidic, neutral ph + high oxygen, enterococci, lactobacilli si, low ph + low oxygen, numerous bacteria li, neutral ph + zero oxygen. Anaerobic bacteria that digests (cid:862)(cid:374)o(cid:374)-digesti(cid:271)le(cid:863) (cid:272)o(cid:373)plex pla(cid:374)t polysaccs (non-starch. They produce different end products and gases. Produce vitamins, essential butyrate for epithelial cells.