MIMG 102 Lecture Notes - Lecture 9: Animal Virus, Rna-Dependent Rna Polymerase, Rna Virus

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 102
• Professor: Dasgupta Asim

Document Summary

Lecture 9 animal virus ctd: how to tell if virus is or + rna virus: Phenol extraction isolates mrna, put mrna inside the cell or in vitro -> the one translated/infectious is +rna: +rna viruses replicase makes/copies -strand (because want more +rna for progeny virus rna) Primary translation of +rna -> viral proteins including replicase. Making many + strands: same replicase copies strands to make many + strand. Carries transcriptase in viral particle -> makes +rna. Translate newly made +rna -> more transcriptase. More transcriptase make + strand to make more -strand for progeny virus. Transcriptase (no +rna => no protein) (q: is there a secondary transcription???: + rna retrovirus. Carries reverse transcriptase (rna-dependent dna pol & dna-dependent dna pol activities; makes dna strands) + strand rna doesn"t act like mrna (doesn"t get translated right away) Rt makes dna copy of +rna -> dsdna. Viral dna then integrated into host dna -> provirus e. g. hiv.