MIMG 102 Lecture Notes - Lecture 14: Chorioallantoic Membrane, Influenzavirus B, Influenza A Virus
20 Mar 2019
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Document Summary
Influenza: characters here typically applicable to other + & - strand viruses, no long-lasting vaccine; virus evolving every year, segmented -strand rna enveloped viruses. Each genome encodes for 1 protein; or 2 proteins from splicing: 3 types of viruses. Genus influenzavirus a 8 genome segments. Genus influenzavirus b - 8 genome segments. Genus influenzavirus c - 7 genome segments, no neuraminidase. Intensity of symptoms differs greatly depending on virus strain. Key development & concepts: virus isolated from ferrets, virus grown chorioallantoic membrane of embryonated chicken eggs, hemagglutination (clumping red blood cells, replication in tissue culture: dependence on host nucleus, host gene expression. If take nucleus out of cell, flu can"t replicate in cell. Steals cap from mrna, which is in nucleus. Grow in de-nucleated cells -> no plaques: pa, pb1 & pb2 are polymerases. Each has to be bound to rna (has to make +strand: ha (hemagglutinin) & na (neuraminidaes) important for receptor recognition.
