MIMG 102 Lecture Notes - Lecture 22: Rolling Circle Replication, Herpesviridae, Dna Replication
20 Mar 2019
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Ori: 3 ori, 2 copies of oris, 1 copy of oril, oril important for productive infection, functional in productive vs latent replication, rolling circle - origins only required for initial stage of replication. Subsequent viral replication is origin-independent: ul9 (origin recognition protein) binds palindromic sequences & recruits viral dna pol, helicase unwinds dna, ul9 is later cleaved by host protease (rest of replication is ori independent) E. g. viral dna pol, ssdna binding protein. E. g. thymidine kinase (tk), ribonucleotide reductase (rnr): these enzymes are required for dntp synthesis. Tk puts phosphate on dtmp and dtdp -> dttp, incorporated into nascent dna. Ribonucleotide reductase rehydrolyzes dntp when they"re not functional for use: early proteins encoded by virus to help facilitate replication. Mimicking host proteins that already exist; viruses copy them to use for their own replication: drug: acyclovir antiviral guanine analog (particularly uses hsv tk to get incorporated into viral dna)
