MCD BIO 165A Lecture Notes - Lecture 6: Profilin, Formins, Wild Type
20 Apr 2017
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What kind of actin-based structures are reduced in the arpc2 knockdown (e. g. figures. Does this make sense given the known function of arp2/3? (2 point) This does make sense because the known function of arp2/3 is to nucleate actin filaments and promote branching to create the lamellipodia structures. If it is knocked down, you will not see that: a) what question were the authors trying to address by using the smifh2 drug in. And, in essence, are formins used to create f-actin structures in the absence of arp2/3: what was the result of this experiment and how do the authors interpret it? (2 points) Inhibiting formins decreased wildtype cell motility but in mutant arpc2 motility is not decreased. What do the results show and what hypothesis did this finding suggest? (2 points) They found that both vasp and mena are expressed in higher amounts in the knockout than in comparison to the wild type cells.
