DGS 3222 Lecture Notes - Lecture 4: Cytogenetics, Nuclear Membrane, Chromosome
2 Sep 2016
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Avery welch medical cytogenetics dgs 3222 week 3 exam extra credit video notes. Spurbeck video notes dynamics of chromosome spreading. Effect of humidity and temp on chromosome spreading. As fixative evaporates, cytoplasm/chromosomes get wider: break open and spread. Long evaporation can cause broken and tight metaphases. The culture and harvest methods the only thing changing is humidity: pha stimulated blood culture, excess thymidine, ethidium bromide, colcemid, hypotonic solution, fixative, temp 25c, vent 15% open, by-pass gate 40% open. 45-55% relative humidity perfect for blood karyotyping. 35% rh tight metaphases; cells drying too fast: excess overlap characteristic of drying too fast but also can occur at high humidities. 60-65%+ relative humidity: chromosomes all float away because it"s too wet, chromosomes end up refractile/hollow. shiny.
