DGS 3223 Lecture Notes - Lecture 2: Cytogenetics, Deconvolution, Eyepiece
8 Sep 2016
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Focusing controls: used to raise and lower the specimen stage to focus the image of the specimen (coarse and fine focus) The lock ensures objective does not come in contact with specimen, simplifies focusing. Label left; wherever the lines on the two rulers" exactly line up: england finder/field finder (pictured right under specimen stage photo) If upside down, it would be labeled h12 usd. Only remove a slide on objectives less than 100x: lowering the stage not necessary. Inverted microscope (pictured right below england finder: objectives below stage, view growing specimens in dishes or flasks. Types of microscopy: transmitted light source in the base of the scope, reflected specimen is illuminated from above, applications. Specimens fixed, mounted, and stained to increase contrast and allow observation. This process kills living cells & therefore we cannot examine live cells with a. Phase plate back focal plane of objective. Ring annulus under lower lens of condenser.
