MCB 2000 Lecture Notes - Lecture 18: Cyclic Adenosine Monophosphate, Pyruvic Acid, Phosphoenolpyruvic Acid

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27 Mar 2017
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(step 6 cont"d) forms covalent bond with cysteine in active site, enzyme usually becomes inhibited, but not in this case. Thioester bond makes and breaks bond; has more energy than atp. Must have enough energy to make 1,3 bpg at end of split. 2 atp produced per glucose; balances out 2 atp input. Using an intermediate with high energy (more energy than atp) to phosphorylate. Reactions being pulled forward based on differences in concentration. Compare ^g, difference between ^go" and ^g is due to concentration differences. Remove a water molecule in this reaction. Phosphorylate adp to atp with enol form of pyruvate. Pyruvate kinase catalyzes reaction, 3rd regulated step in glycolysis. Release more energy here than atp; -31. 4 kj/mol. Results in net 2 atp per glucose. Note difference in free energy change under standard states versus overall free energy change. Know 1,3,6,10 (look at table in slide set) Free energy change based on concentration differences.

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