MCB 2410 Lecture Notes - Lecture 18: Abo Blood Group System, Whole Genome Sequencing, Human Genome Project
17 Dec 2018
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Document Summary
People with bo blood type have nail-patella. Abo blood group locus maps to 9q34. Dna markers or other known sequences can be used to determine clone order to assemble a contig. Use detailed genetic and physical maps to identify the least number of clones to cover the genome (minimum tiling path) Sequence and assemble that small number of clones. Shear entire genome into small pieces and clone. Many large research projects and resources have results or benefitted from the publication of the human genome. Sequencing of millions of nucleotides per run. Thousands of millions of reactions per run. Allows for sequencing of the entire sample. Makes genomes possible (computational speed, power and storage were limiting factors) Millions of short sequencing reads are assembled into contigs based on sequence similarity. Functional genomics - characterize what genome sequences do, and what their function is. Roughly 2% of the human genome is protein coding. Only about half has any known function.
