MCB 2210 Lecture Notes - Lecture 3: Epitope, Immunofluorescence, Immunocytochemistry
11 Sep 2017
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Lecture 3 light microscopy in cell biology: the cells that cell biologists typically study, primary cells, non-cancerous, non-transformed, can be isolated and cultured to study cell-specific functions. Some model basic cell functions, others retain specialized functions. They neither reflect nor absorb much light. + u(cid:374)less the(cid:455)(cid:859)re (cid:272)olored, like (cid:272)hloroplasts, or stained. Contrast is poor and little detail can be made out. Contrast is the difference in intensity between an object and its background: to bring out detail (enhance contrast), we need to exploit changes in the phase of light or stain the object to make it darker. Lecture 3 light microscopy in cell biology: dependent on the properties of light & how it interacts with the specimen, not directly related to magnification. Magnification must be high enough though: determined by. If you see a 200nm spot in a microscope, it could be. Lecture 3 light microscopy in cell biology. Important methods in cell bio that utilize antibodies.
