BME 80G Lecture Notes - Lecture 2: Nanopore Sequencing, Single-Nucleotide Polymorphism, Nanopore
23 Sep 2018
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8/7/18: recap: ed green, dna can communicate information from generation to generation. Y chromosome is paternally inherited, mitochondria are maternally inherited: sequencing technology, moore"s law: higher complexity at reduced cost and time, illumina sequencing. Low error rate, low cost, lots of data, short read length: cut dna into pieces, allows to attach them again on a flo cell to make copies of those dna molecules to amplify it, nanopore sequencing. Thousand base pair fragments, higher error rate, has the potential to identify methylation and acetylation patterns. The current changes depending on what molecule passes through the pore: merging technologies. Single-cell sequencing: cells are uniquely tagged with a barcode in a small oil bubble. Shows potentially different mutations: origins of variation, genetic recombination. You get a random assortment of genes from your mom and dad: crossover event happens during meiosis between the sister chromatids. 99. 9% of animal species reproduce sexually: migration of individuals.
