BIEB 166 Lecture 7 (WI13)

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University of California - San Diego
Biol/Ecology, Behavior, & Evol
BIEB 166
James Nieh

Lecture 7 Knockout Studies with Mice - Block LTP with Drugs (inhibitors) rats have decreased ability to learn spatially - Too general to block their learning ability 1. alpha CaMKII mutants - Silva, A.J. et al. (Tonegawa lab at MIT) Science 257: 206 (1992) - Hippocampal expression a. Long Term Potentiation (LTP) ○ Strengthening of connections (synapses) between nerve cells involved in memory ○ Hippocampus contains a kinase (alpha CaMKII) that controls spatial learning of the mice ○ Morris Water Task  Look for the platform  Water-type mice take less time to reach the platform ○ Mutant mice largely normal but inferior at the hidden platform test b. Problems with approach: i. Kinase may have multiple effects ii. Mutant mice may have problems swimming Huerta, Patricio T. et al. (1996) - Spatial learning in fyn tyrosine kinase knockout mice - Knockouts can learn but have difficult swimming 2. Mayford, M. et al. (Kandel lab at Columbia) Science 274: 1678 (1996) a. Forebrain specific promotor combined with tetracycline transactivator system (feeding tetracycline temporarily suppresses CaMKII) b. Provided both regional and temporal control of transgenic expression c. Could use this promotor to switch alpha CAMKII transgene on and off in adult animal by feeding it tetracycline ○ Only act on hippocampus Learning - A change in behavior as a result of experience Types of learning 1. Adaptation (neurophysiology) - A sensory receptor continually stimulated for a prolonged period stops responding 2. Non-associative learning 2. Non-associative learning - Events are not associated a. Habituation ○ The reduction or elimination of a behavioral response to a stimulus repeatedly presented without positive or negative reinforcement ○ More than just a sensory neuron ○ Ignorance of the stimulus Turkey - Strong negative stimulus by hawks - Ducks do not provide negative stimulus to the turkey b. Dishabituation ○ Therecovery of a habituated response following the presentation of a strong or novel stimulus ○ Can only occur after habituation Aplysia - Every time touching it, it contract immediately, and as poking goes on, the response is weaker - If you
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