BIMM 100 Lecture Notes - Lecture 18: Repressor, Lactose Permease, Ribosome-Binding Site

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The way the cell reads the instructions determines its function: gene expression is regulated to respond to environmental impacts and control metabolism. Gene expression regulated at multiple levels: dna goes through transcriptional regulation to generate pre-mrna. Regulation of mrna in the cytoplasm creates the protein: major regulation is transcriptional regulation. Each separate gene has its own rna sequence as a ribosomal binding site before atg. Lactose is a bigger sugar: galactose + glucose combined: to digest lactose, need 2 enzymes: protein lactose permease on cell membrane to transport lactose into the cell. Lactose digested by beta-galactosidase, which breaks lactose into galactose and glucose. Cap to dna: how lactose represses laci, lactose directly binds to laci protein, so laci cannot bind to dna. This represses the repressor, to activate gene expression. If glucose + and lactose , glucose will repress cap binding and laci will bind to the gene.

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