BIMM 100 Lecture Notes - Lecture 20: Dna-Binding Domain, Chromatin Remodeling, Deoxyribonuclease

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Cis-regulatory elements (promoters and enhancers) are sequences along dna that affect gene transcription. They do so by interacting with trans-regulatory elements (transcription factors) Dna binding domain and activation domain are independent from each other: can take dna binding domain of gene 1 and link it to activation domain of gene 2. These will regulate transcription of gene 1 since the dna binding domain binds to gene 1, but activation domain still works to affect gene 1 transcription. Dna binding domain binds to dna through: binding specificity: protein bind depending on bases of nucleotides, binding affinity: phosphates, sugars, and bases of nucleotides contribute. One nucleosome is 150 bp of dna wrapped around 8 histones (2 of each histone h2a,

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