BIMM 120 Lecture Notes - Lecture 15: Dna Polymerase Iii Holoenzyme, Minimum Inhibitory Concentration, Dna Polymerase I
26 Dec 2018
School
Department
Course
Professor
Document Summary
Induce prophage (piece of dna integrated in bacterial chromosome, dormant until induced by excision -> replication -> virus formation: shiga toxin (shigella"s) transferred via this process. Antibiotic resistance genes can be transferred in the same way. Integrative and conjugative elements that carry antibiotic resistance genes can be transferred to others through conjugation. Then, increase concentration of antibiotics 10-fold over the minimum inhibitory concentration, and see that the bacteria can still grow. Are humans responsible for antibiotic resistance: antibiotic resistance prevalent in isolated cave microbiome: isolated from human contamination, lechuguilla cave isolated from anthropogenic use of antibiotics and antibiotic contamination through water for 4 million years. Identified gram positive and negative resistant bacteria: found that 3 streptomyces were highly resistant to daptomycin (impairs cell membrane polarization, affecting transport of things through cell, daptomycin introduced in 2003 as man- made antibiotic. Found sample looked like microbial sample from soil.
