ANFS300 Lecture Notes - Lecture 8: Micrococcal Nuclease, Chromatin, Agarose Gel Electrophoresis
25 Sep 2020
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Please answer the question below in the text box provided. Histones are associated with the acidic dna backbone in the chromatin: define micrococcal nuclease. Micrococcal nuclease is a bacterial enzyme that is often used for chromatin studies. The smallest band will be about 145 base pairs: gel concentration can also be used to separate dna fragments. The higher the concentration of agarose gel, the smaller the pore size. So high concentrations of agarose are used to separate short fragments of dna and low agarose concentrations resolve larger fragments of dna. Dark bands mean that there are more dna fragments within the gel: the a260 to a280 ratio for a purified dna sample is 1. 3. Carbohydrates, lipids and rna: an a260 of 1. 0 indicates the dna sample contains 50 ug/ml of dna. The absorbance of a purified dna sample at 260 nm is 0. 263. Give your answer in scientific notation in ug per ml.
