ANFS332 Lecture Notes - Lecture 12: Avian Influenza, Hemagglutination, Influenza A Virus
4 Feb 2020
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Document Summary
The sticking of red blood cells to other cells which have been infected with certain types of haemagglutinating viruses. Many viruses attach to molecules present on the surface of red blood cells. As a consequence of this a viral suspension may bind together (agglutinate)the red blood cells thus preventing them from settling out of suspension. The hemagglutination (ha) test measures the amount of hemagglutinin (virus) in a sample. The hemagglutination inhibition (hi) test is a modification which measures the amount of antibody to influenza. The basis of the hi assay is that antibodies to influenza virus will bind to the virus and prevent attachment of the virus to red blood cells. Therefore hemagglutination is inhibited when antibodies are present. The highest dilution of serum that prevents hemagglutination is called the. If the serum contains no antibodies that react with the virus, then hemagglutination will be observed in all wells.
