BISC300 Lecture Notes - Lecture 7: Superoxide Dismutase, Reactive Oxygen Species, Intermolecular Force
21 May 2018
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Lecture 7: Effects on Microbial Growth (continued)
Clicker Questions
1. Two containers A (15 degrees C) and B (5 degrees C)
a. Molecules in A are moving faster than molecules in B
2. What happens that results in a denaturation of a protein?
a. Intermolecular forces (IMFs) break apart
3. Factors that may cause denaturation of a protein
a. High heat
b. High salt concentration
c. pH different from optimal pH
d. dehydration: removal of water will disrupt H-bonds
4. compounds that are prone to denaturation
a. polypeptide
b. nucleic acid
5. Diatomic oxygen is
a. Required for the growth of some living beings
b. Is harmful to some living beings
6. Oxygen is directly involved in:
Temperature
• are bacteria homeotherms or poikilotherms?
• Why is temp important?
• Cardinal temperatures
• Temp ranges for microbial growth
o Highest temperature will have the most profound effect on denaturation
• Adaptations of thermophiles
o What adaptations
▪ Protein
▪ DNA
▪ Membrane
Basis of Different Oxygen Sensitivities
• O2 reduced to reactive oxygen species
o Superoxide radical
o Hydrogen proxide
o Hydrolxyl radical
• Aerobes produce protective enzymes
o Superoxide dismutase (SOD)
o Catalyase
o Peroxidase
Strict Anaerobes
• Obligate
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