BISC300 Lecture Notes - Lecture 5: Exponential Growth, Microbiological Culture, Gram-Negative Bacteria
21 May 2018
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Lecture 5: Bacterial Growth
Clicker Review
1. Positive chemotaxis: net directional movement toward a chemical stimulus
2. Storage inclusions can store:
a. Glycogen
b. Amino acids
c. Gas
3. Only some bacterial species have flagella
4. Bacterial endospores contain one copy of bacterial genomic DNA
5. Primary function of bacterial endospore: overcome unfavorable environment conditions
6. Structure of endospore is more similar to Gram negative cell
a. Outer membrane
Bacterial Cell Cycle
1. Initiation mass reached
2. Initiation of replication
3. Origins separate
4. Cell elongates as chromosome replication and partitioning continue
5. Septum formation begins when Z ring forms
6. Chromosomes separate as separation continues
7. Cells divide
Have identical DNA
Growth curve in closed system
• [utriet] … dynamics of bacterial
growth
• Growth rate constant ~ generation time
• Practical uses of knowing about
dynamics of bacterial growth
• In batch culture (closed system)
• Lag phase: release enzymes, acclimate
to environment
• Exponential phase is from exponential
growth
• Stationary phase: generation of waste products, no more nutrients to consume
• Decline phase
o Carrying capacity
o Mutations may make a cell able to consume materials that cells were no longer
able to
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