MEDT220 Lecture Notes - Lecture 12: Oral Sex, Glycoprotein, Amylose
4 Oct 2017
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Hemoglobin required for a positive presumptive test for blood. In noncompetitive capture elisa, the color produced is directly proportional to the amount of analyte in the sample. Approximately 20-30% of human proteins are polymorphic (multiple alleles) Polymorphisms: hb a, hb s (sickle cell), hba1c (diabetic complications) Complex mixture of enzymes (acid phosphatase) and proteins like (prostate specific antigen) No sperm present (might have had a vasectomy) Acid phsophatase test would be used in this case. Alternative light source (als): 450-495 nm or 350-380 nm. Remove paper circle from stain and spray with. Sap liberates naphthol from alpha-naphthol and the naphthol reacts to form a purple colored dye. Any substance with acid phosphatase activity (watermelon, vaginal fluid) Main value in determining blood type is to exclude a suspect. Buccal epithelial cells (sse: simple squamous epithelial cells) Iodine reacts with starch to form a purple color. Amylase breaks down starch to mono/disaccharides which do. Not form purple color in presence of iodine.
