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Lecture 28

CHM 3218 Lecture Notes - Lecture 28: Cytoplasm, Citric Acid Cycle, Lyase


Department
Chemistry
Course Code
CHM 3218
Professor
Stewart Jon
Lecture
28

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isocitrate Lyase
Aldol .
coi
goi =-
oty 't +(oi
ozcyvcoi :
if
.it#i*
'
t.EE#0** Oo¥t+¥I⇐ty⇐¥
'
Mi "I't
Qyoxalate
Aldol
0t
yg.hydrolysis 0Hydrolysis -0 ,
11 =Ytcoi + ( OASH
/\s(oA + Jd NH + HZO OH
same mechanism
oh
as citrate synthase to _)~o
Glucose
to
Ho 'At bit
-02C NADH µglucoheogehesis
Ylco ,
" "#%
at
-tfoi #mµj0iY"
oi
Oxalo acetate protein Cytosol Oxaloautste
for glyoxalcte
shunt is transported
Into cytosol as Asp
so that enzymes of
gluloheogenesis cannot
utilize it .
Regulation of Citric Acid Cycle
1
Pyruvatl DH NAD 't NADH
Coast
wioi ,q¥y%n*
Glucose →→ #a,
→_ Ig. a
citrate
TAcetyl -CoA
Is the fuel
Cannot use HEB to °f a. him acid cycle
determined
thus 't is regulated
Calculated so "= -z KI
mole
Covalent Regulation
(EI kinase )
ATP ADP
-
E@~oH -E@ to .
§
-o
-
Active -b-
MInactive
pi Hi
phosphatase ( regulated by Coit levels )
Ayoskric modulators of EI kinase
NADH , #sort 9increase activity
ADP ' %cos f Decrease activity
Glyoxalate Shunt ( bacteria )Isoitrte Dehydrogenase ( IDH )
-
no compartmentalization
pp§nAD*
iiyfiioiuotyuoi
IN #
phosphorylation
(low km
(high km )ICI
otygtt Three
-km regulation results
ICDH being preferred
entym it present
since higher affinity
and phosphorylation
is
2nd level of regulation
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