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Lecture 6

MCB 3020 Lecture 6: Microbiology Lecture 6 Exam One

3 Pages

Microbiology and Cell Science
Course Code
MCB 3020
Abdolkarim Asghari

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9/9/2017 Microbiology lecture 6—Exam one • Bacterial endospores o Formed by some bacteria o Dormant o Resistant to numerous environmental conditions ▪ Heat radiation ▪ Chemicals ▪ Desiccation • What makes an endospore so resistant?: o Calcium (complexed with dipicolinic acid) o Small acid-soluble, DNA-binding proteins o Dehydrated core o Spore coat o DNA repair enzymes o Sporulation vs. germination (vegetative conditions. Normal. When conditions become harsh, they become endospore) o Spore is produced by fungi for reproduction • Plasmids can be shared via conjugation • Peptidoglycan is unique to bacteria. In form of pseudopeptidoglycan. • Life is based on the universal principle of SPECIFIC INTERACTION OF MOLECULES WITH EACH OTHER. Interacting molecules interact as pairs are called LIGAND and RECEPTOR. • Ligand and receptor—where a lot of things come to life. Sugars are part of the carbohydrate;they act like receptors on surface of cell • Glycolipids (sugar attached to lipids) and glycoproteins (sugar attached to proteins) act like receptors Culture Media • Most contain all the nutrients required by the organism for growth • Defined or synthetic—all components are known • Complex—plant, animal or yeast extract. No specific ingredient; just a protein in general. Don’t know all components • Selective—only one group grows. EX: MacConky agar—only ram negative can grow, not positive • Differential • Common o Macroelements (macronutrients) o C, O, H, N, S, P, K, Ca, Mg, and Fe o Micronutrients (trace elements) o Mn, Zn, Co, Mo, Ni, Cu • Mechanisms of Action of Selective and Differential Media o Blood agar. Three types of reactions: Alpha, Beta (completely destroys or lysis cells) and Gamma (hemolysis—kinda destroys. Functional type—enriched and differential o Eosin methylene blue (EMB) agar. Functional type: selective and dif
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