MCB 4320C Lecture 2: Lectures 2-4
19 Jan 2019
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Document Summary
To understand the microbiome, you must learn the technologies used to study it. Learn the steps and usefulness of the four dominant sequencing technologies used today. Know when it is appropriate to use one versus another. Know the amounts of dna sequence they can produce in a given period of time. Know the current uses of sanger sequencing. Know why it is no longer used for genome sequencing. Know why it remains an important technology today. Not uncommon for phd thesis to include several completely closed bacterial sequences and their comparative analysis. Oldest method in use today: sanger sequencing. 1980 - base sequencing of rna and dna. Dominated all dna sequencing from 1977 - 2005. Still used today as a very effective and inexpensive means to obtain a few sequences. No longer used for high throughput sequencing as it has been supplanted by more cost effective methods beginning in 2005.
