MCB 2000 Lecture Notes - Lecture 6: Endospore, Facilitated Diffusion, Osmotic Pressure

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Lecture 6
Cell membranes
o Cell membranes are selectively permeableonly let certain things in and out
o Cell membrane has fluid mosaic model
The membrane is not rigid
o Simple diffusion
o Facilitated diffusion through a nonspecific transporter
o Facilitated diffusion through a specific transporter
o Osmosis through an aquaporin
Osmotic pressure = the pressure that would have to be applied to a pure
solvent to prevent it from moving into a given cell
Usually use to express the concentration of the soln
Osmosis = movement of water into and out of a cell
o Movement of water depends on what is on the outside of the cell
o Isotonic = water conc on inside and outside is same
Cell remains same shape
No movement of water
o Hypotonic = water conc on inside of cell is lower than on outside
Solute concentration on inside of cell is higher
Water moves into cell
Cell swellsmay burst
Cell walls help keep the cells from bursting
o Hypertonic = water conc on inside is higher than on outside
Solute concentration on inside of cell is lower
Water moves out of cell
Cell shrinks
In high salt and sugar solns, bacteria cannot growwhy we pickle things
and make jams/jellies
Endospores
o Some bacteria can go into a dormant state when conditions become too harsh =
endospore
o Steps in forming an endospore = sporulation
Spore septum begins to isolate newly replicated DNA and small amt of
cytoplasm
Plasma membrane starts to surround new DNA, cytoplasm and
membrane isolated in step 1
Spore septum surrounds isolated portionforms forespore
Peptidoglycan layer forms between membranes
Spore coat forms
Endospore is freed from cell
o When conditions normalize, cell becomes a vegetate through germination to
become active again
o Clostridium and Bacillus do this
Bacterial cell overview
o External structures
Appendages
Flagella (movement)
Pilli (attachment)
Fimbriae (attachement)
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