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Lecture 6

MCB 3020 Lecture 6: Microbiology Lecture 6 Exam One

3 Pages
22 Views
Fall 2017

Department
Microbiology and Cell Science
Course Code
MCB 3020
Professor
Abdolkarim Asghari
Lecture
6

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9/9/2017
Microbiology lecture 6Exam one
Bacterial endospores
o Formed by some bacteria
o Dormant
o Resistant to numerous environmental conditions
Heat radiation
Chemicals
Desiccation
What makes an endospore so resistant?:
o Calcium (complexed with dipicolinic acid)
o Small acid-soluble, DNA-binding proteins
o Dehydrated core
o Spore coat
o DNA repair enzymes
o Sporulation vs. germination (vegetative conditions. Normal. When conditions become
harsh, they become endospore)
o Spore is produced by fungi for reproduction
Plasmids can be shared via conjugation
Peptidoglycan is unique to bacteria. In form of pseudopeptidoglycan.
Life is based on the universal principle of SPECIFIC INTERACTION OF MOLECULES WITH EACH
OTHER. Interacting molecules interact as pairs are called LIGAND and RECEPTOR.
Ligand and receptorwhere a lot of things come to life. Sugars are part of the
carbohydrate;they act like receptors on surface of cell
Glycolipids (sugar attached to lipids) and glycoproteins (sugar attached to proteins) act like
receptors
Culture Media
Most contain all the nutrients required by the organism for growth
Defined or syntheticall components are known
Complex—plat, aial or east etract. No specific igrediet; just a protei i geeral. Do’t
know all components
Selectiveonly one group grows. EX: MacConky agaronly ram negative can grow, not positive
Differential
Common
o Macroelements (macronutrients)
o C, O, H, N, S, P, K, Ca, Mg, and Fe
o Micronutrients (trace elements)
o Mn, Zn, Co, Mo, Ni, Cu
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Description
9/9/2017 Microbiology lecture 6—Exam one • Bacterial endospores o Formed by some bacteria o Dormant o Resistant to numerous environmental conditions ▪ Heat radiation ▪ Chemicals ▪ Desiccation • What makes an endospore so resistant?: o Calcium (complexed with dipicolinic acid) o Small acid-soluble, DNA-binding proteins o Dehydrated core o Spore coat o DNA repair enzymes o Sporulation vs. germination (vegetative conditions. Normal. When conditions become harsh, they become endospore) o Spore is produced by fungi for reproduction • Plasmids can be shared via conjugation • Peptidoglycan is unique to bacteria. In form of pseudopeptidoglycan. • Life is based on the universal principle of SPECIFIC INTERACTION OF MOLECULES WITH EACH OTHER. Interacting molecules interact as pairs are called LIGAND and RECEPTOR. • Ligand and receptor—where a lot of things come to life. Sugars are part of the carbohydrate;they act like receptors on surface of cell • Glycolipids (sugar attached to lipids) and glycoproteins (sugar attached to proteins) act like receptors Culture Media • Most contain all the nutrients required by the organism for growth • Defined or synthetic—all components are known • Complex—plant, animal or yeast extract. No specific ingredient; just a protein in general. Don’t know all components • Selective—only one group grows. EX: MacConky agar—only ram negative can grow, not positive • Differential • Common o Macroelements (macronutrients) o C, O, H, N, S, P, K, Ca, Mg, and Fe o Micronutrients (trace elements) o Mn, Zn, Co, Mo, Ni, Cu • Mechanisms of Action of Selective and Differential Media o Blood agar. Three types of reactions: Alpha, Beta (completely destroys or lysis cells) and Gamma (hemolysis—kinda destroys. Functional type—enriched and differential o Eosin methylene blue (EMB) agar. Functional type: selective and dif
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