MCB 3020L Lecture : ELN Lab 8 5-23 Combating Microbes (cameron).docx

• School: University of Florida
• Department: Microbiology and Cell Science
• Course: MCB 3020L
• Professor: ashgar

Document Summary

The purpose of this lab is to learn how to use the kirby-bauer antibiotic disk. By successfully completing this lab, we should be able to understand how antibiotics affect microbes, and which antibiotics work best on certain microbes. It can be hypothesized that there are certain antibiotics that are successful at preventing growth of gram positive bacteria, and other antibiotics that work on gram negative bacteria. There are some microbes that may be resistant to many different types of bacteria. Apply the antibiotics individually to the surface with forceps dipped in alcohol and flamed. Make sure that contact is made with the surface. Do not remove the antibiotic once it has. Label the bottom of each mueller-hinton agar plate with your initials, date, medium type and test microorganism. Using aseptic technique, inoculate each plate with their respective test organisms as above. Take a sterile empty filter disk with sterile tweezers.