ADSC 3130 Lecture Notes - Lecture 2: Rodent, Miscarriage, Lysine
13 Sep 2017
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They will go to genes of interest and activated them: for gene expression, you can perform rt-pcr (polymerase chain reaction), quantitative rt-pcr, rna-sequencing, different types of rna: mrna, trna, mirna, shrna. For our class, we are usually talking about mrna: protein expression, this is done to further define, you can do things like immunocytochemistry, flow cytometry, western blots. Functional tests (basically, does the cell do what it"s supposed to do) adult stem cells will. Embryo body test (1 functional test)- take embryonic stem cells, and make a large mass of them. This is done in scid mice (they are immunodeficent). After the stem cells are injected under the skin, the mice will get huge masses. Not as accurate: in-vivo (happens in body)- stronger test. Chimera assay (3 functional test) (one of his favorites- remember: developing blastocyst, you inject into your blastocyst.
