ADSC 3130 Lecture Notes - Lecture 1: Mesenchymal Stem Cell, Facial Nerve Paralysis, Rodent

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Animal Biotech
10/17/2017
Systemic administration of exosomes released from mesenchymal stromal cells promote
functional recovery and neurovascular plasticity after stroke in rats
The Problem: Stroke
2nd leading cause of death over 60 years old
2 types of strokes:
Ischemic stroke- restriction of blood flow; a clot blocks blood flow to an area of the
brain.
Hemorrhagic stroke- bleeding occurs inside or around brain tissue.
The Solution: Exosomes
Cell to cell communication system
o Play a role in intracellular signaling and mediating immune response
o Creates an immune response
Exosomes contain mRNA, proteins, DNA, miRNAs, cytokines, lipids
o Can get from bone marrow, skin, etc.
Therapeutic potential for strokes
Exosomes can cross blood brain barrier to help bring in things to help with strokes
Hypothesis: systemic administration of MSC (mesenchymal stem cells) derived exosomes post
stroke will improve functional outcomes.
After a stroke, you wouldn’t want to give orally (facial paralysis, can’t swallow), don’t give it in
the muscle because the muscle is far from brain.
MSC (mesenchymal stem cells)
Can get these very easily
Functional outcomes: walk, talk, feed themselves
Methods:
Mesenchymal stromal cells from bone marrow of a rat
Behavior tests
o Foot fault test
Start box, and mouse
You expect mouse to go across horizontal latter
o Neurological score
Measures neurological deficient
Lesion volume measuring
o H&E staining: a stain to quantify the outcomes of post-stroke
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