CBIO 3400 Lecture Notes - Lecture 6: Copii, Amphiphile, Sec61
6 Mar 2019
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Today"s class: er to golgi traffic, n-glycosylation in the golgi. Paper from breakout will be on exam 3. *review the crosslinking experiment (question 4) from breakout before friday. Budding of vesicles from the er: coat proteins coat the vesicle (associate with the cytoplasmic side of the golgi). They physically deform the membrane: physically create a bud and select cargo that will go on, first type of coat protein- copii, comes from coatamer proteins, sec 23/24, sec 13/31. Sar1 is a gtpases, so we can expect that it has a gap and a gef: second type we characterize, copii- goes from er to golgi. Copii: sar1 curves the er membrane, copii is monomeric & has gap and gef regulation. Important for controlling the assembly of the copii-coat. In gtp bound state, sar1 will release an amphiphilic helix which is part of the protein.
