GENE 3200 Lecture Notes - Lecture 3: Single-Nucleotide Polymorphism, Tandem Repeat, Variable Number Tandem Repeat
Document Summary
Fine scale genetic maps: very few polymorphic visible markers. With these maps, we are limited by on the affected people we can see (can"t see the non-affected carriers). Either a c or a t at this snp: large # allows saturation of genome. Snp arrays: short ssdna molecules to a flat surface in known positions (dna microarray, this is similar to a southern blot. Label and hybridize sample dna to array: measure hybridization to score genotype, on a clip, there are strands of dna that are bolted down to the glass. Snp vs str: snp is the variable base, only use snps that have 2 alleles, homozygous for the g or t and heterozygous for tg, follow mendel"s rules, equal segregation. If unlinked, independent assortment: str- number of cac repeats, markers used for things such as forensics because they are so variable. Range from 10,11,12,13: use primers flanking these.
