GENE 3200 Lecture Notes - Lecture 5: Human Genome Project, Genomic Library, Sequence Assembly
Document Summary
The human genome project: genomics: the content, organization, function, and evolution of genetic information contained in whole genomes, motivation, find disease genes (forward genetics, determine number, identity, and function of loci in genome (reverse genetics) Clone by clone (map-based) sequencing: like positional cloning except across the entire genome*, large clones (~150 kb, use yeast and bacterial artificial chromosomes, sequence each bac: Identify genome location of each clone using mapping: fragment dna and sub-clone into plasmids (~1 kb, assembled sequence, overview: divide the genome into large clones and clone it using bacs (bacterial clones). Then, make a genomic library and figure out the bacs you need to make up the entire human genome. Whole genome shotgun (wgs) sequencing: new sequencing technique, cut entire genome into small fragments (1-12 kb) clone fragments sequence clone ends assemble, lots of sequencing (10-20x coverage of each site, disadvantages, computationally very intensive.
