BCHS 3304 Lecture Notes - Lecture 19: Enzyme Kinetics, Turnover Number, Rate Equation

• School: University of Houston
• Department: Biochemistry
• Course: BCHS 3304
• Professor: Yeo Hye- Jeong

Document Summary

Lecture 19: when the concentration of s is much higher than e. Rate of enzyme is independent of the substrate concentration. The reaction is of zero order: michaelis menten equation. Ks= [e][s]/[es: ks= dissociation constant, es= michaelis complex. As the michaelis complex peaks, the free enzyme concentration dips. They reach a steady state where they stay constant: at this time [e] and [es] are both constant. Km is substrate concentration where the velocity of the reaction is half of the maximum. If small km = max velocity at low substrate concentration. As ks decreases, km decreases and affinity: catalytic constant= kcat increases. Turnover number= catalytic reactions per active site per time. When the substrate concentration is much higher than the enzyme concentration. Rate of enzyme is independent of substrate. Chemical kinetics concentration: chemical or enzymatic reactions take place at different rates. Rate= progress of the reaction as time passes.