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CHEM 237 (10)
M.C.White (10)
Lecture 3

Chem 237, Lecture 3.pdf

2 Pages

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CHEM 237

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Column chromatography to purify compounds ⿞Alternative to recrystalization;used for small quantities because it is more effective and for liquids that cannot recrystalize • Make sure you use silica gel in the hood!!! • Make slurry of silica gel using your column solvent,whichever solvent mixture will promote the best separation in samples on aTLC plate • Agitate the column to release trapped air in the slurry,then pack column with slurry by applying air (do not push all the solvent through though,keep it above the silica gel level,don't run the column dry) • Top the silica gel with sand,keep the layer thin • Prepare your compound as a suspension in as little possible of the column solvent as possible • Bring the solvent level below the sand,and slowly add the compound suspension on to the sand layer • Apply a little air pressure to load the compound onto the silica • Rinse the compound flask with a little solvent,and add to the column,then again load onto the silica ⿞Repeat multiple times to ensure all the compound is loaded onto the silica • Load with solvent to begi
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