MCB 354 Lecture Notes - Lecture 12: Allosteric Regulation, Cooperative Binding, Covalent Bond
Document Summary
Amount of enzyme (vmax proportional to [etot] Reversible non covalent binding of reg molecule (allosteric modulator) A few key enzymes can set the rate (flux) of multienzyme pathway. Some enzymes are obvious choices because they catalyze rds or energy intensive steps. Often earliest dedicated step is regulated by being specifically inhibited by the end product of the pathway. This negative feedback control slows the whole pathway and is called: The end product of a pathway is usually very different from the s of the regulated enzyme (allosteric enzymes) Allostery - control of e/protein activity by multiple ligands that bind distinct, non-overlapping sites and affect each other"s affinity or reactivity over a distance. Allostery means other shape - ligands that bind at distinct sites may have entirely different structures. A ligand that affects the activity of an allosteric protein is an allosteric regulator/modulator/effector. Can be inhibitors - often the end product of the pathway.