BIO 152 Lecture Notes - Lecture 5: Meiosis, Mucus, Ribosome

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lavendergoat171

12 Apr 2017

School

University of Kentucky

Department

Biology

Course

BIO 152

Professor

Seth Jones

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Document Summary

Osmolarity = osmotic concentration: solute amount (osmole) per liter (osm/l, pure water is 0 osm/l. Same number of moles for most substances: exception: compounds that dissolve when dissolved in water, example: 1 mole nacl dissociates into 1 mole na+ and 1 mole cl-, so there are. 2 osmoles of nacl in 1 mole nacl. Multiple compounds can make up osmotic concentration: 3 osm solution = 1 mole glucose + 1 mole nacl (2 osm nacl, 3 osm solution of glucose/nacl = 3 osm solution of 3 mole glucose. Water will diffuse (osmosis) from lower osmolality to higher osmolality. On side a of the membrane is 1m glucose. Comparison between solute concentration inside the cell vs outside the cell: unlike osmolality, tonicity ignores solutes that can freely diffuse across the cell membrane. Hypertonic solution more (hyper) solutes outside the cell than inside the cell: example: very salty water.

Related Questions

1. In lecture, we described a simple osmotic pressure gradient when discussing osmosis. For question #1, describe the simple osmotic pressure gradient, in terms of osmotic pressure, that exists across the red blood cell plasma membrane when red blood cells are suspended in a severely hypotonic solution, such as a 0.2% NaCl solution. Use scientific terminology.

2. We mentioned in class that biological systems are always in a state of osmotic equilibrium due to a combination of solute diffusion in some cases and osmosis in others. In this experiment, when you add the red blood cells to a severely hypotonic solution, what substances are moving across the red blood cell plasma membrane to achieve osmotic equilibrium, Na+ and Cl- or water? Explain why based on your understanding of the permeability characteristics of biological membrane. Always use scientific language.

CAN QUESTIONS 1 AND 2 BE ANSWERED DIRECTLY AND ADEQUATLEY PLEASE.

HERE IS AN INTRO TO THE LAB EXPERIMENT

Intro:

We have known for a very long time that the mammalian red blood cell plasma membrane is very permeable to water. Even the introductory biology student understands that if you put mammalian red blood cells in water, they will rapidly swell and break open (hemolyze) due to the rapid osmosis of water into the cell. The experimental question we are asking in today's lab experiment is "Is the high water permability of mammalian red blood cell plasma membrane due solely to the lipid bilayer, or are there water channels present in the membrane?"

Experimental Design:

It has been shown by several investigators that water movement through water channels can be blocked by the known K+ channel blocker, tetraethylammonium chloride (TEAC). Brooks, Regan and Yool (2000) showed that TEAC reduces the water permeability of human water channels expressed in the plasma membranes of frog ocytes. After preincubation of the oocytes for 15 min with 100 microM TEAC, the water permeability of frog oocyte plasma membrane containing human water channels was reduced 20 to 40%. The investigators concluded that treatment of cells with TEAC can be useful in determining the presence of aquaporins in membranes.The specific objective of today's experiment is to uncover the presence of water channels in mammalian red blood cell plasma membrane by determining the effect of tetraethylammonium chloride on the water permeability of mammalian RBC plasma membrane.

I have this questions for my homework i need help with them look please it is important i need help so please give me the correct answers for each question and also please be clear to understand and aslo be correct it is very important becuase i need them so i really need the right answers. please help me and on Questions 6 and 9 please help me with it hope you can help me with the correct answers please

Question 5

At a constant temperature, if you increase the number of unsaturated fatty acid chains in the phospholipid, the membrane will become......

less fluid

more fluid

fluidity will be unchanged

Question 6

Bacteria in a hot spring at 80 degrees celsius would be expected to have ______________ in their cell membrane

high proportions of saturated phospholipids

high proportions of unsaturated phospholipids

an equal ratio of saturated to unsaturated phospholipid

Question 7

Membrane proteins have an important role in membrane fluidity

True

False

Question 8

The HIV virus can't attach to cells that have a mutation in the CCR5 gene and so such individuals are resistant to HIV. What population of people have a higher incidence of the mutant CCR5 gene and why?

europeans because their ancestors survived the bubonic plague

North Americans because their ancestors survived small pox

europeans because they have a longer evolutionary past with HIV

Africans because they have a longer evolutionary past with HIV

Question 9

Nematode trapping fungi have ______________ molecules called ______________ that recognize specific carbohydrates on the surface of the nematode worms. This is how a fungus can recognize that its prey, the nematode worm, has wriggled into the loop. Write two words on lowercase separated by a space with no other syntax

Question 10

Diffusion requires energy

True

False

Question 11

The net direction of molecule movement when molecules diffuse is

down their concentration gradient

random directions

Question 12

In osmosis, water molecules from a solution of higher solute concentration cross a semi-permeable membrane into a solution of lower solute concentration?

True

False

Question 13

Which best defines osmosis?

In osmosis, water from a solution of lower solute concentration move across a semi-permeable membrane into a solution of higher solute concentration?

In osmosis, water from a solution of higher solute concentration move across a semi-permeable membrane into a solution of lower solute concentration?

Question 14

Animal cells placed into a hypotonic solution would most likely?

lyse (burst)

shrink

remain unchanged in size

Question 15

Glucose and amino acids move into cells via carrier proteins, the same protein can move glucose or amino acids

True

False

Question 16

ATP powered pumps move substances into and out of cells by diffusion?

True

False

Question 17

For every 3 Na ions moved out, 2 K ions are moved into a cell by one cycle of the sodium-potassium pump.

True

False

Question 18

Bulk transport of fluid and any of the substances within that fluid is called

pinocytosis

phagocytosis

receptor-mediated endocytosis

exocytosis

Question 5

At a constant temperature, if you increase the number of unsaturated fatty acid chains in the phospholipid, the membrane will become......

less fluid

more fluid

fluidity will be unchanged

Question 6

Bacteria in a hot spring at 80 degrees celsius would be expected to have ______________ in their cell membrane

high proportions of saturated phospholipids

high proportions of unsaturated phospholipids

an equal ratio of saturated to unsaturated phospholipid

Question 7

Membrane proteins have an important role in membrane fluidity

True

False

Question 8

europeans because their ancestors survived the bubonic plague

North Americans because their ancestors survived small pox

europeans because they have a longer evolutionary past with HIV

Africans because they have a longer evolutionary past with HIV

Question 9

Question 10

Diffusion requires energy

True

False

Question 11

The net direction of molecule movement when molecules diffuse is

down their concentration gradient

random directions

Question 12

In osmosis, water molecules from a solution of higher solute concentration cross a semi-permeable membrane into a solution of lower solute concentration?

True

False

Question 13

Which best defines osmosis?

In osmosis, water from a solution of lower solute concentration move across a semi-permeable membrane into a solution of higher solute concentration?

In osmosis, water from a solution of higher solute concentration move across a semi-permeable membrane into a solution of lower solute concentration?

Question 14

Animal cells placed into a hypotonic solution would most likely?

lyse (burst)

shrink

remain unchanged in size

Question 15