BIOLOGY 173 Lecture Notes - Lecture 7: Restriction Enzyme, Hindiii, Restriction Site
Document Summary
M/t (13th & 14th)- no labs: w/th (15th & 16th)- mol bio 2, m/t (20th & 21st)- mol bio 2; w nite (22nd)- mol bio 3 demo, w/th (22nd & 23rd)- mol bio 3, f (24th)- quiz 1. Dna ligase: enzyme which makes phosphodiester bonds between pieces of dna, normally joins okazaki fragments during dna replication, scientists can use this enzyme to join two restriction fragments. Restriction endonucleases: bacterial proteins which bind dna at a specific site and cleave a phosphodiester bond, recognition site, usually 6 bp and palindromic example- ecori. In an agarose gel, dna must move through pores in the gel. Dna is stained with ethidium bromide if you put it in an electric field, it will migrate to the positive electrode smaller fragments go through faster smaller fragments will move farther in a given amount of time. Etbr intercalates between bases of dna for this reason etbr is mutagenic.
