MCDB 310 Lecture Notes - Lecture 2: Endonuclease, Restriction Enzyme, Sticky And Blunt Ends

958 views3 pages

Document Summary

Simple biochemical principles and methods are often applied to complex problems in cell and molecular biology. In addition, sometimes research projects take an odd turn, and we need to go back and check our work. We are studying the enzyme lactase, which cleaves the disaccharide lactose into galactose and glucose. Dna sequence of the lactase gene was recombined with a plasmid. We intended to introduce the plasmid into e. coli cells and then follow the expression of lactase from the plasmid gene from the bacteria. This method also has to verify that the gene is free of errors. Automated dna sequencing: (1 point) the result from you method above shows that the authentic lactase gene is present in the plasmid. You now want to test whether the e. coli cells express lactase mrna. Use a different method than the one used for q1.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

Related Questions