MENAS 398 Lecture Notes - Lecture 9: Lactase, Repressor, Plasmid

• School: U of M
• Department: Middle Eastern And North African Studies
• Course: MENAS 398
• Professor: Carol Bardenstein

Document Summary

General instructions: you may work alone or in groups having of up to 4 students. Groups should submit a single copy of their work with the names of all participants on it: deadline for submission is tuesday, 23 february at 5:30 pm (full credit). This ensures that your paper ends up with the correct gsi. Simple biochemical principles and methods are often applied to complex problems in cell and molecular biology. In addition, sometimes research projects take an odd turn, and we need to go back and check our work. We are studying the enzyme lactase, which cleaves the disaccharide lactose into galactose and glucose. A dna sequence of the lactase gene was recombined with a plasmid. We intended to introduce the plasmid into e. coli cells and then follow the expression of lactase from the plasmid gene from the bacteria. This method also has to verify that the gene is free of errors.