MCDB 423 Lecture 24: Lecture 24

2 Pages
Unlock Document

Molecular, Cellular and Developmental Biology
MCDB 423
John Kuwada

Lecture 24 Slide 3 When you delete the Nogo receptors, the axons do grow a little past the injury site Slide 5 Answer = d Answer C was the biggest factor in choosing these genes They choose a handful of factors -Used cre to remove the genes -All these genes are tumor suppressors so they normally restrain growth These cells don’t die because the flox system is removing them only from certain cells -So the animal will have gone through devleopment normally slide 6 The cre is introduced to the adult mice using a virus into the eye -So the RGC will express the cre CTB is used only for labeling of the axons Slide 7 This is a control to see if the method works -This is a reporter to see if the cre is working slide 8 The GFP virus is basically the wild type control because this virus DNA won’t do anything In the mutant, you see many axons past the injury sites Slide 9 Answer = b. They noted some fiber in the optic chiasm, but there is no data for as far out as the SC. The controls are the green and red line -X-axis is distance pass the cut site -As you go pass the cut site, there are very few fibers in the wt -In the mutant there are more fibers slide 10 The other major phenotype after axonal damage is that the neurons die Slide 11 Answer = b. Other mutations (in p53) give increased survival in absence of the regeneration phenotype. So increasing cell survival alone is not sufficient for regeneration. Some of the
More Less

Related notes for MCDB 423

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.