MCDB 423 Lecture Notes - Lecture 35: Nuclear Localization Sequence, Cell Membrane, Olfactory Receptor Neuron

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 423
• Professor: John Kuwada

Document Summary

Serially sectioned the entire worm and reconstructed the entire cns. Inserted a microelectrode into one neuron and then insert another microelectrode into another neuron. Then see if the two neurons are synaptically related by stimulating one neuron while recording from the other neuron. If they are synaptically related, you should be able to record a synaptic potential. This is the best way to demonstrate a synaptic relationship. Multiple methods have been used as a proxy for electrophysiology. Right eye projects to the left ot and vice versa. A transgenic fish was made that expressed gcamp. It is a (cid:373)odified gfp (cid:449)here (cid:271)(cid:455) itself, it does(cid:374)"t fluores(cid:272)e as (cid:373)u(cid:272)h (cid:449)hereas a (cid:374)or(cid:373)al gfp fluroesces a lot. It"s tagged o(cid:374) the n-terminus with a subunit of the myosin light chain called m13 and on the c- terminus with a protein called calmodulin (cam) O(cid:374)e of the thi(cid:374)gs ca(cid:373) does is that it (cid:271)i(cid:374)ds ca so it"s a ca dete(cid:272)tor.